Journal: PLOS ONE

Article Title: CXCL12 impact on glioblastoma cells behaviors under dynamic culture conditions: Insights for developing new therapeutic approaches

doi: 10.1371/journal.pone.0315038

Figure Lengend Snippet: GBM: Glioblastoma multiforme; Alg 1%: Alginate hydrogel 1%; Alg/Chit-NPs CXCL12: Alginate/Chitosan-Nanoparticles loaded with CXCL12; Alg:M: Alginate:Matrigel (50:50); CXCL12: C-X-C motif chemokine 12.

Article Snippet: Human recombinant CXCL12 (SDF-1α) (purity ≥98%, PeproTech United States, Rocky Hill, NJ, United States) was reconstituted in sterile Milli-Q water following the manufacturer’s instruction.

Techniques:

Journal: PLOS ONE

Article Title: CXCL12 impact on glioblastoma cells behaviors under dynamic culture conditions: Insights for developing new therapeutic approaches

doi: 10.1371/journal.pone.0315038

Figure Lengend Snippet: (a) Schematic representation of the perfusion bioreactor system for GBM cells migration assay. (b) The perfusion bioreactor assembly. GBM: Glioblastoma multiforme; Alg 1%: Alginate hydrogel 1%; Alg/Chit NPs-CXCL12: Alginate/Chitosan-Nanoparticles loaded with CXCL12; Alg:M: Alginate:Matrigel (50:50); PDMS: Polydimethylsiloxane; 3D: three-dimensional.

Article Snippet: Human recombinant CXCL12 (SDF-1α) (purity ≥98%, PeproTech United States, Rocky Hill, NJ, United States) was reconstituted in sterile Milli-Q water following the manufacturer’s instruction.

Techniques: Migration

Journal: PLOS ONE

Article Title: CXCL12 impact on glioblastoma cells behaviors under dynamic culture conditions: Insights for developing new therapeutic approaches

doi: 10.1371/journal.pone.0315038

Figure Lengend Snippet: (a) Hydrogels scans of mCherry-F98 GBM cells migration at t = 0 h, t = 24 h and t = 72 h with and without CXCL12 (0.33 μg) under a flow rate of 0.5 μL/min. (b) Hoechst staining of the hydrogels at t = 72h. (c) DNA quantification of the different hydrogel sections at the end of the experiment (N = 15, n = 3). Bars represent the mean ± SD and the squares are zoomed regions. Statistical analysis was performed using a Mann-Whitney t-test, * p ≤ 0.05, ** p ≤ 0.01. The scale bar represents 2000 μm. NPs-Empty: Alginate/Chitosan-Nanoparticles unloaded; NPs-CXCL12: Alginate/Chitosan-Nanoparticles loaded with CXCL12; Alg:M: Alginate:Matrigel (50:50); Alg 1%: Alginate 1% hydrogel.

Article Snippet: Human recombinant CXCL12 (SDF-1α) (purity ≥98%, PeproTech United States, Rocky Hill, NJ, United States) was reconstituted in sterile Milli-Q water following the manufacturer’s instruction.

Techniques: Migration, Staining, MANN-WHITNEY

Journal: PLOS ONE

Article Title: CXCL12 impact on glioblastoma cells behaviors under dynamic culture conditions: Insights for developing new therapeutic approaches

doi: 10.1371/journal.pone.0315038

Figure Lengend Snippet: (a) Hydrogels scans of mCherry F98 GBM cells migration at t = 0 h, t = 24 h and t = 72 h with and without CXCL12 at a flow rate of 3 μL/min. (b) Hoechst staining of the hydrogels at t = 72 h. (c) DNA quantification of the different hydrogel sections at the end of the experiment (N = 9, n = 3). Bars represent the mean ± SD and the squares are zoom regions. Statistical analysis was performed using a Mann-Whitney t-test, * p ≤ 0.05, ** p ≤ 0.01. The scale bar represents 2000 μm. NPs-Empty: Alginate/Chitosan-Nanoparticles unloaded; NPs-CXCL12: Alginate/Chitosan-Nanoparticles loaded with CXCL12; Alg:M: Alginate:Matrigel (50:50); Alg 1%: Alginate 1% hydrogel.

Article Snippet: Human recombinant CXCL12 (SDF-1α) (purity ≥98%, PeproTech United States, Rocky Hill, NJ, United States) was reconstituted in sterile Milli-Q water following the manufacturer’s instruction.

Techniques: Migration, Staining, MANN-WHITNEY

Journal: PLOS ONE

Article Title: CXCL12 impact on glioblastoma cells behaviors under dynamic culture conditions: Insights for developing new therapeutic approaches

doi: 10.1371/journal.pone.0315038

Figure Lengend Snippet: (a) Hydrogels scans of U87-GFP GBM cells migration at t = 0 h, t = 24 h and t = 120 h with and without CXCL12 at a flow rate of 0.5 μL/min. (b) Hoechst staining of the hydrogels at t = 120 h. (c) DNA quantification of the different hydrogel sections at the end of the experiment (N = 9, n = 3). Bars represent the mean ± SD and the squares are zoom regions. Statistical analysis was conducted using a Kruskal-Wallis test followed by Dunn’s multiple comparison post-hoc test, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. 1 : (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12) *, (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12+) *, (Alg:M + cells NPs-CXCL12 vs. Alg:M + cells NPs-CXCL12+) ns. 2 : (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12) ns, (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12+) **, (Alg:M NPs-CXCL12 vs. Alg:M NPs-CXCL12+) ns. 3 : (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12) ns, (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12+) ***, (Alg:M NPs-CXCL12 vs. Alg:M NPs-CXCL12+) ns. 4 : (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12) ns, (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12+) ns, (Alg:M + cells NPs-CXCL12 vs. Alg:M + cells NPs-CXCL12+) **. The scale bar represents 2000 μm. NPs-Empty: Alginate/Chitosan-Nanoparticles unloaded; NPs-CXCL12: Alginate/Chitosan Nanoparticles loaded with CXCL12; Alg:M: Alginate:Matrigel (50:50); Alg 1%: Alginate 1% hydrogel.

Article Snippet: Human recombinant CXCL12 (SDF-1α) (purity ≥98%, PeproTech United States, Rocky Hill, NJ, United States) was reconstituted in sterile Milli-Q water following the manufacturer’s instruction.

Techniques: Migration, Staining, Comparison

Journal: PLOS ONE

Article Title: CXCL12 impact on glioblastoma cells behaviors under dynamic culture conditions: Insights for developing new therapeutic approaches

doi: 10.1371/journal.pone.0315038

Figure Lengend Snippet: (a) Hydrogels scans of GFP U87 GBM cells migration at t = 0 h, t = 24 h and t = 120 h with and without CXCL12 at a flow rate of 3 μL/min. (b) Hoechst staining of the hydrogels at t = 120 h. (c) DNA quantification of the different hydrogel sections at the end of the experiment (N = 9, n = 3). Bars represent the mean ± SD and the squares are zoom regions. Statistical analysis was conducted using a Kruskal-Wallis test followed by Dunn’s multiple comparison test, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. 1 : (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12) ns, (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12+) **, (Alg:M + cells NPs-CXCL12 vs. Alg:M + cells NPs-CXCL12+) ns. 2 : (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12) ns, (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12+) **, (Alg:M NPs-CXCL12 vs. Alg:M NPs-CXCL12+) ns. 3 : (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12) ns, (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12+) ***, (Alg:M NPs-CXCL12 vs. Alg:M NPs-CXCL12+) ns. 4 : (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12) ns, (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12+) ns, (Alg:M + cells NPs-CXCL12 vs. Alg:M + cells NPs-CXCL12+) **. The scale bar represents 2000 μm. NPs-Empty: Alginate/Chitosan-Nanoparticles unloaded; NPs-CXCL12: Alginate/Chitosan-Nanoparticles loaded with CXCL12; Alg:M: Alginate:Matrigel (50:50); Alg 1%: Alginate 1% hydrogel.

Article Snippet: Human recombinant CXCL12 (SDF-1α) (purity ≥98%, PeproTech United States, Rocky Hill, NJ, United States) was reconstituted in sterile Milli-Q water following the manufacturer’s instruction.

Techniques: Migration, Staining, Comparison

Journal: Immunology and Cell Biology

Article Title: RNAi library screening reveals Gβ1, Casein Kinase 2 and ICAP‐1 as novel regulators of LFA‐1‐mediated T cell polarity and migration

doi: 10.1111/imcb.12838

Figure Lengend Snippet: Pathway analysis of the hits identified from the kinome and GPCR/GPCR‐associated RNAi library screens.

Article Snippet: Recombinant human SDF‐1α (CXCL12) and IL‐2 were from Peprotech (London, UK).

Techniques: Activation Assay, Virus, Infection